WEBINÁŘ: Thermo Scientific Proteome Discoverer Phosphopeptides Online Training Workshop

Phosphopeptide analysis is a critical aspect of understanding cellular signaling and disease mechanisms, yet it comes with its own set of challenges. Phosphopeptides are often present in low abundance compared to their non-phosphorylated counterparts, requiring an enrichment step as part of the sample preparation protocol. Phosphopeptides can be difficult to ionise and fragment efficiently in mass spectrometry, and specific instrument method settings should be employed to address that. Data analysis and interpretation is complicated as well, due to the presence of multiple phosphorylation sites on a single peptide and the potential for different phosphorylation states (e.g., mono-, di-, or tri-phosphorylation). 

Addressing these challenges involves optimising sample preparation protocols, using advanced mass spectrometry techniques, and employing robust bioinformatics tools to ensure accurate and comprehensive phosphopeptide analysis. This workshop will provide you with valuable insights and practical solutions to overcome these hurdles, enhancing your research capabilities.

What You Will Learn:

• Strategies for enriching low-abundance phosphopeptides
• Techniques to minimize phosphate group loss during sample preparation
• Advanced mass spectrometry methods for efficient phosphopeptide detection
• Best practices for data interpretation and bioinformatics analysis
• Case studies with hands-on data processing examples

Speakers:

You are unable to attend the webinar broadcast on September 17? Please register anyway to automatically receive the link to the recording.