WEBCAST: Structural lipidomics using Real-Time Library Search
Or, how mass spectrometry with on-the-fly software improves phospholipid characterization
Date: Thursday, March 30, 2023
Time: 8AM PDT | 11AM EDT | 4PM BST | 5PM CEST
In mass spectrometry-based lipidomics, complex lipid mixtures undergo chromatographic separation, ionization, and detection using high resolution, accurate mass (HRAM) mass spectrometry to simultaneously quantify and structurally characterize eluting species. The reported structural variety of identified lipids is strongly reliant on the analytical techniques utilized.
This webcast will present a novel lipidomics Real-Time Library Search (RTLS) approach on an Orbitrap Tribrid mass spectrometer that uses real-time lipid class identifications to trigger class-targeted MSn to improve the structural characterization of glycerophospholipids and sphingomyelins in the positive ion mode. This class-based RTLS method demonstrates improved selectivity compared to current methodology, enhancing lipid characterization applications.
You will learn:
- How recent technology advancements improve lipid analysis
- What type of samples can be analysed using on-the-fly mass spectrometry
- Tips and tricks to gaining more confident lipid annotation
Presenters:
- Dr. Dain Brademan , Postdoctoral Fellow, Morgridge Institute for Research University of Wisconsin–Madison
- Moderator: Nikki Forrester , Freelance science writer and editor
Produced by: Presented by:
