WEBINARS Thermo Scientific Orbitrap Astral

The recently unveiled ultra-fast scanning Thermo Scientific Orbitrap Astral mass spectrometer is a game-changer for comprehensive proteome coverage and high-throughput biomedical applications. In this webcast, Dr. Jesper V. Olsen of the University of Copenhagen will describe the capabilities in data-independent acquisition (DIA) mode with narrow quadrupole isolation windows in combination with short LC gradients.

Register to learn:

• Benefits of narrow-window data-independent acquisition (DIA)
• How to acquire comprehensive human proteomes within hours
• Optimize methods for low input samples, including single cells

• Jesper V. Olsen Ph.D , Novo Nordisk Foundation, Center for Protein Research, University of Copenhagen

OnDemand Webinar: Single-cell proteomics on the Orbitrap Astral MS for the exploration of cell heterogeneity

Single-cell proteomics by mass spectrometry can provide valuable insights into distinct cell states and signaling patterns present in a cell population. However, carrying out proteomics profiling from the limited amount of material encapsulated in an individual cell presents significant challenges.

Tremendous efforts have been made to optimize all aspects of single-cell proteomics by mass spectrometry, with the aim of minimizing losses during sample preparation and maximizing sensitivity of data acquisition. Nevertheless, continuous development is needed to increase both the quantifiable proteome depth per cell, the quantitative accuracy and precision of those measurements, and the number of cells measured per unit time.

This webcast will present recently-developed approaches, ranging from sample preparation and chromatographic separation to advanced data acquisition, data interpretation and visualization methods. Advances in instrumentation, including the novel Orbitrap Astral MS, and how to use both TMTPro-labeled and label-free approaches, will be presented, showcasing the application of these methods to biomedical questions, and providing an overview of the current technological state of the field.

Learn how to:

• Prepare single cells for analysis by mass spectrometry
• Improve the sensitivity and throughput of single-cell proteomics analysis
• Implement both label-free and labeled quantitative approaches to single-cell proteomics

On-Demand Webinar: How Mass Spectrometry Innovation Propels Biological Discovery

Accurate, quantitative, high-throughput multi-omics measurements can fill longstanding gaps in basic biochemistry and elucidate confounding disease processes. This webcast will highlight how mass spectrometry innovations enable deep proteome sequencing and population-scale biological discovery.

Learn how to:

• Study biological systems using multi-omics approaches.
• Perform deep proteome sequencing using mass spectrometry.
• Apply novel mass spectrometry technology to solve biological questions.

• Josh Coon , Professor of chemistry and biomolecular chemistry, University of Wisconsin-Madison

Host Cell Protein (HCP) Profiling and Quantitation in Gene Therapy Products with the Orbitrap Astral Mass Spectrometer

Learn how you can achieve confident, sensitive and reproducible AAV HCP identification.

This webinar will demonstrate approaches to overcome key challenges with AAV HCP analysis, such as the highly complex proteome background and large dynamic range which necessitate the need for high sensitivity enabling the detection and quantification of low abundant HCPs, essential for safe and efficacious production of these advanced biotherapeutics.

In this webinar you’ll learn:

• How to overcome challenges in HCP analysis of AAV based gene therapies
• How to ensure you accurately profile and quantify critical HCP contaminants in the sub ppm range
• How the new Thermo Scientific Orbitrap Astral Mass Spectrometer can aid your HCP analysis

Speaker:

• Jonathan Bones , Principal Investigator, Characterization and Comparability Group, NIBRT